A thiouracil desulfurase protects Clostridioides difficile RNA from 4-thiouracil incorporation, providing a competitive advantage in the gut

2025/03/27 by Matthew J. Munneke, Yifeng Yuan, Eva C. Preisner +18 · 1 voice · 6 citations
Biochemistry, Genetics and Molecular Biology · Medicine · #Biochemical and Molecular Research #Biology #Cell biology #Clostridioides #Clostridium difficile and Clostridium perfringens research #Endocrinology #Microbiology #Thiouracil #Thyroid #Viral gastroenteritis research and epidemiology

paper · open access · doi:10.1016/j.chom.2025.03.001

published in Cell Host & Microbe 33(4), 573-588.e7 (Cell Press)

openalex publication_date 2025/03/27 · crossref created 2025/03/27 · crossref issued 2025/04/01 · crossref published 2025/04/01 · crossref published-print 2025/04/01 · crossref deposited 2025/06/09 · openalex created_date 2025/10/10 · crossref indexed 2026/05/12 · openalex updated_date 2026/08/01

Citations

• Pfam: The protein families database in 2021
• AutoDock Vina 1.2.0: New Docking Methods, Expanded Force Field, and Python Bindings
• Highly accurate protein structure prediction with AlphaFold
• Introducing the Bacterial and Viral Bioinformatics Resource Center (BV-BRC): a resource combining PATRIC, IRD and ViPR
• Interactive Tree of Life (iTOL) v6: recent updates to the phylogenetic tree display and annotation tool
• RudS: bacterial desulfidase responsible for tRNA 4-thiouridine de-modification
• 4-thiouridine inhibits rRNA synthesis and causes a nucleolar stress response
• Open Babel: An open chemical toolbox
• Evolved bacterial resistance against fluoropyrimidines can lower chemotherapy impact in the Caenorhabditis elegans host

Cited by

• Metabolic ecology of microbiomes: Nutrient competition, host benefits, and community engineering

Discussions

• 🔬 New research reveals how Clostridioides difficile gains a competitive edge in the gut: a thiouracil desulfurase detoxifies 4-thiouracil, converting it to uracil and preventing harmful RNA incorpora [bsky, 0 points, 0 comments]