2025/04/08 by Romain Lannes, Jaclyn M. Fingerhut, Yukiko Yamashita · 1 voice
Biochemistry, Genetics and Molecular Biology · #DNA and Biological Computing
paper · pdf · doi:10.1101/2025.04.06.647416
openalex publication_date 2025/04/08 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/14
Abstract We report the development of a framework-free program to assess splicing events: OmniSplice. This new method comprehensively identifies the end of every exon, and categorizes the RNA sequence read based on the sequence that follows the exon ends. OmniSplice detects not only ‘spliced’ and ‘nascent’ reads but also unpredicted reads. Using this method, we detected aberrant splicing events, including backsplicing that we failed to detect in our previous analysis upon depletion of a splicing factor U2AF.