OmniSplice: a framework-free splicing event reporter

2025/04/08 by Romain Lannes, Jaclyn M. Fingerhut, Yukiko Yamashita · 1 voice · 2 citations
Biochemistry, Genetics and Molecular Biology · #Biology #Computational biology #Computer science #DNA and Biological Computing #Event (particle physics) #Gene #Genetics #Physics #RNA splicing

paper · pdf · doi:10.1101/2025.04.06.647416

published in bioRxiv (Cold Spring Harbor Laboratory) (Cold Spring Harbor Laboratory)

openalex publication_date 2025/04/08 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/14

Abstract

Abstract We report the development of a framework-free program to assess splicing events: OmniSplice. This new method comprehensively identifies the end of every exon, and categorizes the RNA sequence read based on the sequence that follows the exon ends. OmniSplice detects not only ‘spliced’ and ‘nascent’ reads but also unpredicted reads. Using this method, we detected aberrant splicing events, including backsplicing that we failed to detect in our previous analysis upon depletion of a splicing factor U2AF.

Citations

• Recursive splicing in long vertebrate genes
• Circular RNAs: Characterization, cellular roles, and applications
• Origin and evolution of spliceosomal introns

Discussions

• OmniSplice: a framework-free splicing event reporter doi.org/10.1101/2025... Genome-wide analyses supported by RNA-Seq reveal non-canonical splice sites in plant genomes doi.org/10.1101/428318 [bsky, 0 points, 0 comments]

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