2024/12/17 by Tutku Aykanat, Athina Balatsou, Kirsi Kähkönen +6 · 1 voice
Biochemistry, Genetics and Molecular Biology · Environmental Science · #Fish Ecology and Management Studies #Genetic diversity and population structure #Identification and Quantification in Food
paper · pdf · doi:10.1111/jfb.16032
openalex publication_date 2024/12/17 · openalex created_date 2024/12/18 · openalex updated_date 2026/08/01
A workflow for developing a cost- and time-efficient, single nucleotide polymorphism (SNP)-based assay for species and hybrid identification is described. In a reference set (n = 46), the developed assay identified individuals of two closely related species, the Atlantic salmon (Salmo salar L., n = 23) and brown trout (Salmo trutta, n = 23), with 100% accuracy. Furthermore, species and hybrid identification using field-collected embryos had 98.1% concordance (155/158) to more expensive and time-consuming methods that utilized multiple SNP markers. The method can be integrated into management and conservation plans to quantify species' spawning distribution and hybridization rates.