2025/05/16 by Jingbo Yang, Guofang Zhang, Xinyu Wang +6 · 1 voice
Agricultural and Biological Sciences · Nursing · #Infant Nutrition and Health #Microbial Metabolites in Food Biotechnology #Probiotics and Fermented Foods
paper · pdf · doi:10.3168/jds.2025-26429
openalex publication_date 2025/05/16 · openalex created_date 2025/05/17 · openalex updated_date 2026/07/25
Human milk oligosaccharides (HMO) play an important role in the growth and development of infants. Some bifidobacteria have been shown to use HMO through conserved and strain-specific pathways and also have shown different physiological characteristics under different carbon sources. Previous studies have concentrated on representative strains of each genus, yet there is a paucity of research on the metabolic and function differences between strains within subspecies. In this work, we compared the differences between Bifidobacterium infantis CICC 6069 and B. infantis Z-081 under glucose, lactose, galactooligosaccharides, and 2'-fucosyllactose (2'-FL), including growth and metabolic, gastrointestinal digestive resistance, and adhesion to Caco-2. Compared with the type strain CICC 6069, B. infantis Z-081 had higher tolerance characteristics under different carbon sources, but its adhesion characteristics were lower than those of CICC 6069. And it was proved that 2'-FL could promote the adhesion of B. infantis. Furthermore, the distinct global gene transcription response was observed among the strains. The results of this study provide a comprehensive understanding of the genomic differences between 2 strains of B. infantis, ideas for the screening of functional probiotics, and further support the potential application of 2'-FL as a prebiotic supplement.