2024/05/24 by Syed Muhammad Saad Imran, Seth Wiley, Carolyn E. Lubner · 1 voice
Engineering · Chemistry · Environmental Science · #Electrochemical sensors and biosensors #Electrochemical Analysis and Applications #Microbial Fuel Cells and Bioremediation
paper · doi:10.1016/j.coelec.2024.101536
openalex publication_date 2024/05/24 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/25
Flavin-based electron bifurcation (FBEB) was discovered as a significant process of microbial energy conservation less than two decades ago. Since then, several classes of enzymes engaging in FBEB have been identified, all of which utilize a flavin cofactor that accepts two electrons and then transfers one along an exergonic (high-potential) pathway and the other along an endergonic (low-potential) pathway. We describe the critical role of electrochemical techniques, especially protein-film voltammetry and spectroelectrochemistry, in determining the mechanism and energetic landscape of FBEB in a characteristic enzyme. A prospectus of future directions involving currently unutilized electrochemical techniques is discussed with regards to the salient open questions in the field of FBEB.