2025/06/20 by Marcos Luis‐Calero, Federica Marinaro, Canuto Muñoz‐García +7 · 1 voice
Biochemistry, Genetics and Molecular Biology · Medicine · #Renal and related cancers #Reproductive Biology and Fertility #Sperm and Testicular Function
paper · doi:10.1016/j.anireprosci.2025.107946
openalex publication_date 2025/06/20 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
In vitro embryo production is widely used in equine clinical reproduction; however, its efficiency remains suboptimal due to the undermined developmental competence of in vitro-matured oocytes. In this work, we evaluated the effect of mare preovulatory follicular fluid (PFF) secretome supplementation during in vitro maturation (IVM) of equine oocytes recovered post-mortem. Secretome was isolated from PFF and was added to the IVM medium at concentrations of 20 µg/ml (S20) or 40 µg/ml (S40). The percentage of mature oocytes, as well as embryo cleavage, blastocyst development, and gene expression of embryos produced by intracytoplasmic sperm injection (ICSI) was analysed. Gene expression of embryos at day 7 post-ICSI was analysed by real-time quantitative PCR. We observed that the addition of PFF secretome at 40 µg/ml (S40) significantly increased oocyte maturation rates (Control vs. S40: 35.4 ± 3.6 % vs. 46.5 ± 2.7 %; mean % ± SEM; P < 0.05) and reduced the proportion of degenerated oocytes after maturation (Control vs. S40: 58.6 ± 4.2 % vs. 44.0 ± 4.8 %; P < 0.05) compared to the control group. However, no effects were observed on cleavage or blastocyst rates, nor on the expression of the candidate genes studied, namely BEX2, FABP3, ODC, MOBKL3, HSP90AA1, BAX and BCL2. In conclusion, supplementation of PFF secretome during IVM at 40 µg/ml, but not at 20 µg/ml, improves the meiotic competence of post-mortem retrieved equine oocytes but does not influence their developmental competence or the expression of the selected genes in the resulting embryos.