2016/03/01 by Anette Engesmo, Wenche Eikrem, Sergio Seoane +5
Biochemistry, Genetics and Molecular Biology · Earth and Planetary Sciences · Environmental Science · #Marine Biology and Ecology Research #Marine Bivalve and Aquaculture Studies #Protist diversity and phylogeny
paper · doi:10.2216/15-115.1
crossref issued 2016/03/01 · crossref published 2016/03/01 · crossref published-print 2016/03/01 · openalex publication_date 2016/03/01 · crossref created 2016/04/22 · crossref published-online 2019/03/21 · crossref deposited 2023/08/17 · openalex created_date 2025/10/10 · crossref indexed 2026/07/28 · openalex updated_date 2026/07/29
:Heterosigma akashiwo is one of the smallest raphidophytes and is known for its ability to form dense, fish-killing blooms. In many parts of the world, H. akashiwo is a permanent resident of the algal community, and blooms have caused extensive financial losses for the aquaculture industry. Different aspects of its biology have been the focus of numerous investigations over the last two decades but, perhaps because of its small size and fragile nature, few morphological studies have been carried out. We conducted phylogenetic and morphological studies of 24 strains of Heterosigma from the coastal waters of Europe, North America, Australia and New Zealand. Complete 18S, ITS region and the D1–D2 region of 28S rDNA were sequenced and compared with all available raphidophyte sequences, revealing a novel species of Heterosigma, Heterosigma minor sp. nov. All strains of H. akashiwo were genetically very similar across the rDNA region (> 99.8% similarity) and morphologically very similar in light microscopy and scanning electron microscopy. Heterosigma minor shared only 98.2% similarity with H. akashiwo, and there were several morphological differences: it was smaller, rounder and had fewer mucocysts than H. akashiwo. The secondary structure of ITS2 was reconstructed, revealing three compensatory base changes between the two species, further indicating that H. minor is a sister to H. akashiwo. The phylogeny of Raphidophyceae was reconstructed to include all currently described raphidophytes.