2005/01/01 by M. Navarrete, M Navarrete, Santos Casado +10
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Bee Products Chemical Analysis #Insect and Pesticide Research #Spectroscopy Techniques in Biomedical and Chemical Research
paper · doi:10.1080/00218839.2005.11101151
crossref issued 2005/01/01 · crossref published 2005/01/01 · crossref published-print 2005/01/01 · openalex publication_date 2005/01/01 · crossref published-online 2015/03/24 · crossref created 2016/06/29 · crossref deposited 2020/09/23 · openalex created_date 2025/10/10 · openalex updated_date 2026/03/25 · crossref indexed 2026/07/27
SUMMARYCoelectroosmotic capillary zone electrophoresis has been employed for the rapid analysis of 8 aliphatic acids usually present in honey. Polycationic surfactants (hexadimethrine bromide (HDB), cetyltrimethylamonium bromide (CTAB) and tetradecyltrimethyllammonium bromide (TTAB)) have been added to the electrolyte buffer dynamically coating the inner surface of the capillary and causing a fast anodic electroosmotic flow, known as coelectroosmotic flow. The composition of the running buffer (50 mM sodium phosphate adjusted to pH 8 with 1.0 M sodium hydroxide, 25% 2-propanol and 0.001% HDB) and the instrumental parameters (voltage of −20 kV with a hydrodynamic injection for 15 s and an UV detection at 210 nm) have been optimized. Finally, the applicability of the method has been demonstrated for the determination of the aliphatic acids in several kinds of honey without any sample pretreatment.