2024/09/04 by Zhirong Zhu, Shichang Liu, Xupeng Wu +5 · 1 voice
Engineering · Materials Science · Chemistry · #Nanoplatforms for cancer theranostics #Luminescence and Fluorescent Materials #Molecular Sensors and Ion Detection
paper · pdf · doi:10.1002/smo.20240028
openalex publication_date 2024/09/04 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
Abstract The development of efficient aggregation‐induced emission (AIE) active probes is crucial for disease diagnosis, particularly for tumors and cardiovascular diseases. Current AIE‐active probes primarily focus on improving their water solubility to resist aggregation, thereby achieving an initial fluorescence‐off state. However, the complex biological environment can cause undesirable aggregation, resulting in false signals. To address this issue, we have ingeniously introduced an azo group into the AIE luminogen (AIEgen), developing a reductase‐activated AIE probe, Azo‐quinoline‐malononitrile (QM)‐PN, for imaging hypoxic environments. In this probe, the azo group promotes intramolecular motion through rapid E / Z isomerization, causing the excited state energy to dissipate via non‐radiative decay, thus turning off the initial fluorescence. In the presence of reductase, Azo‐QM‐PN is reduced and cleaved to produce the hydrophobic AIEgen NH 2 ‐QM‐PN, which subsequently aggregates and generates an in situ AIE signal, thereby imaging the hypoxic environment with reductase. Encapsulation of Azo‐QM‐PN with DSPE‐PEG 2000 results in the formation of the nanoprobe Azo‐QM‐PN NPs, which can effectively penetrate cell membranes, specifically illuminate tumor cells, monitor fluctuations in azo reductase levels, and deeply penetrate and image multicellular tumor spheroids, demonstrating potential for hypoxic tumor imaging. Additionally, the nanoprobe Azo‐QM‐PN NPs can selectively image hypoxic atherosclerotic plaque tissues, showing potential for detecting atherosclerosis. Therefore, in this study, we successfully developed an enzyme‐activated AIE probe for imaging hypoxic environments, laying the foundation for further clinical applications.