2014/04/18 by Adrian Negrean, Huibert D. Mansvelder · 1 voice · 2 citations
Biochemistry, Genetics and Molecular Biology · Engineering · #Advanced Fluorescence Microscopy Techniques #Optical Coherence Tomography Applications #Photoacoustic and Ultrasonic Imaging
paper · doi:10.1364/boe.5.001588
openalex publication_date 2014/04/18 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
In laser-scanning microscopy often an off-the-shelf achromatic doublet is used as a scan lens which can reduce the available diffraction-limited field-of-view (FOV) by a factor of 3 and introduce chromatic aberrations that are scan angle dependent. Here we present several simple lens designs of superior quality that fully make use of high-NA low-magnification objectives, offering diffraction-limited imaging over a large FOV and wavelength range. We constructed a two-photon laser-scanning microscope with optimized custom lenses which had a near diffraction limit point-spread-function (PSF) with less than 3.6% variation over a 400 µm FOV and less than 0.5 µm lateral color between 750 and 1050 nm.