2025/11/28 by Anna Marianne Weijler, Moritz Muckenhuber, Marlena Muhm +6 · 1 voice
Immunology and Microbiology · Medicine · #Food Allergy and Anaphylaxis Research #Mast cells and histamine #T-cell and B-cell Immunology
paper · pdf · doi:10.1016/j.ajt.2025.11.015
openalex created_date 2025/11/28 · openalex publication_date 2025/11/28 · openalex updated_date 2026/06/19
The emergence of immunoglobulin E (IgE) antibodies specific for donor major histocompatibility complex (MHC) has been observed in murine transplant models and kidney transplant recipients. Although IgE's significance in allergies and other TH2-type diseases is well-documented, its potential role in transplant rejection remains largely unexplored. Besides its involvement in immediate-type allergic hypersensitivity reactions via its high-affinity receptor FcϵRI, IgE augments allergen-specific T cell activation and antibody production through its low-affinity receptor FcϵRII/CD23, a process termed "facilitated antigen presentation (FAP)" Here, we investigate whether donor MHC-specific IgE amplifies allo-immune responses in transplantation through CD23-dependent FAP. Upon rejection of a mismatched cardiac allograft, murine recipients exhibited elevated frequencies of FcεRI+ basophils and IgE+CD23+ B cells in blood and spleen, along with increased levels of IgE binding via FcεRI and CD23. In vitro, donor-specific IgE facilitated the binding of donor MHC antigens to B cells. Employing a footpad immunization model, IgE enhanced T cell activation and proliferation and increased the levels of donor-reactive B and germinal center B cells in draining lymph nodes via a CD23-dependent process. These findings reveal that donor-specific IgE amplifies the alloresponse by promoting the activation and proliferation of donor-reactive lymphocytes through CD23-dependent mechanisms, uncovering a potential role for IgE in transplant rejection.