2026/01/01 by Franziska Walterspiel, Begoña Ugarte-Uribe, Stefan Terjung +3 · 1 voice
Biochemistry, Genetics and Molecular Biology · Chemistry · #Biotin and Related Studies #Click Chemistry and Applications #Molecular Sensors and Ion Detection
paper · pdf · doi:10.1039/d6cb00017g
openalex publication_date 2026/01/01 · openalex created_date 2026/03/04 · openalex updated_date 2026/06/26
and on living cells. We identify a naphthyl-substituted DIO ligand exhibiting superior photoswitching and photoclick efficiency, allowing fast, selective labeling of HaloTagged proteins on the surface of living cells using visible light activation (405 nm). Using this system, we achieve two- and three-color labeling of defined cell surface regions with excellent spatial and temporal precision, additionally allowing combinatorial labeling. Together, this work establishes a versatile framework for multiplexed, light-directed protein labeling compatible with living systems, with promising future applications including multiplexed long-term tracking and cellular barcoding.