2026/03/14 by Laura Carlton, Miray Altınkaynak, Shannon Kelley +7 · 1 voice
Medicine · Biochemistry, Genetics and Molecular Biology · Engineering · #Optical Imaging and Spectroscopy Techniques #Spectroscopy Techniques in Biomedical and Chemical Research #Near-Field Optical Microscopy
paper · pdf · doi:10.1117/1.nph.13.2.025001
openalex publication_date 2026/03/14 · openalex created_date 2026/03/15 · openalex updated_date 2026/08/01
Significance: Diffuse optical tomography (DOT) enables mapping of functional near-infrared spectroscopy channel-based optical density changes to spatial images of oxy- and deoxyhemoglobin. Accurate reconstruction requires optimization for specific probe geometries. Although prior work focused on volumetric voxel reconstructions with grid arrays, here we examine high-density hexagonal arrays for surface-based reconstructions of the brain and scalp. Aim: We evaluate measurement and spatial regularization, spatial basis functions, and reconstruction strategies to reduce crosstalk and improve localization. Both single-wavelength (indirect) and dual-wavelength (direct) approaches are compared. Approach: Simulations with a white-noise model guided parameter optimization using image quality metrics. Resting-state data were augmented with synthetic hemodynamic response functions (HRFs) to incorporate real measurement variance into the parameter optimization pipeline, and results were validated with a ball-squeezing motor task. Results: Gaussian spatial bases reduced brain-scalp crosstalk but lowered contrast-to-noise ratio and increased localization error. Indirect hemoglobin reconstruction decreased oxy-deoxy crosstalk. Validation data showed strong, lateralized motor cortex activation contralateral to the active hand. Conclusions: High-density hexagonal arrays enable accurate surface DOT reconstructions when optimized. Resting-state data augmented with synthetic HRFs provide an effective strategy for parameter selection, yielding localized activation with a high contrast-to-noise ratio.