2026/03/18 by David V. McCalley, S.M.C. Buckenmaier, J. Heaton · 1 voice
Chemistry · Environmental Science · #Analytical Chemistry and Chromatography #Chromatography in Natural Products #Environmental and Analytical Chemistry Studies
paper · doi:10.1016/j.chroma.2026.466904
openalex publication_date 2026/03/18 · openalex created_date 2026/03/20 · openalex updated_date 2026/07/15
The mechanism and potential applications of per-aqueous liquid chromatography (PALC) using highly aqueous non toxic and environmentally friendly mobile phases were investigated using a number of polar stationary phases primarily manufactured for hydrophilic interaction chromatography. Stationary phases were probed with a range of simple acid, neutral and basic solutes of low MW. For charged solutes, ion exchange (IEX) was the dominant retention mechanism; hydrophobic effects and possibly direct adsorption onto the polar column surface also contribute. IEX is a rather neglected mechanism and deserves further study in analytical separations, due to favourable mobile phase properties. Peak shapes were good for adenosine mononucleotides (MW ∼500) despite predictions that poor peak shapes would result from multipoint interactions. Whether PALC should be regarded as a new technique or a combination of classical mechanisms is debated.