ERGA-BGE reference genome of Gambusia holbrooki, a globally invasive freshwater fish

2026/06/04 by Bonnie Fraser, Clelia Gasparini, Francesco Santi +15 · 1 voice
Biochemistry, Genetics and Molecular Biology · Immunology and Microbiology · #Genomics and Phylogenetic Studies #Myxozoan Parasites in Aquatic Species #Aquaculture disease management and microbiota

paper · doi:10.12688/openreseurope.22693.2

openalex publication_date 2026/06/04 · openalex created_date 2026/06/05 · openalex updated_date 2026/07/22

Abstract

<ns5:p> The <ns5:italic>Gambusia holbrooki</ns5:italic> (eastern mosquitofish) reference genome will offer a crucial resource for understanding the evolution and adaptation of invasive freshwater fish species. The genome of <ns5:italic>G. holbrooki</ns5:italic> was assembled into two haplotypes through a phased assembly approach; however, only the primary haplotype was designated as the reference genome for annotation and downstream analyses. The entirety of the genome sequence was assembled into 24 contiguous chromosomal pseudomolecules and 1 mitochondrial genome. This chromosome-level assembly encompasses 0.67 Gb, composed of 421 contigs and 318 scaffolds, with contig and scaffold N50 values of 15.9 Mb and 29.6 Mb, respectively. </ns5:p>

Citations

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• Merqury: reference-free quality, completeness, and phasing assessment for genome assemblies
• Significantly improving the quality of genome assemblies through curation
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• BUSCO update: novel and streamlined workflows along with broader and\n deeper phylogenetic coverage for scoring of eukaryotic, prokaryotic, and\n viral genomes
• Efficient Architecture-Aware Acceleration of BWA-MEM for Multicore Systems
• miRBase: from microRNA sequences to function

Discussions

• Understanding the genetic basis of biological invasions can help improve predictions of how invasive species establish, adapt, and impact native ecosystems. → doi.org/10.12688/ope... #Genomics #Invas [bsky, 0 points, 1 comments]