2026/04/24 by Neel R. Gandhi, Kogieleum Naidoo, Keeren Lutchminarain +25 · 1 voice
Immunology and Microbiology · Medicine · #Diagnosis and treatment of tuberculosis #Immune responses and vaccinations #Tuberculosis Research and Epidemiology
paper · doi:10.1093/ajrccm/aamag140
openalex publication_date 2026/04/24 · openalex created_date 2026/05/02 · openalex updated_date 2026/08/01
RATIONALE: Transmission is the primary driver of tuberculosis (TB) and drug-resistant TB (DR-TB) in high-burden countries; however, where and between whom spread occurs is poorly understood. OBJECTIVES: We conducted universal whole genome sequencing (WGS) to evaluate the role of casual contact in Mycobacterium tuberculosis transmission. METHODS: We recruited persons diagnosed with second-line DR-TB (eg, extensively drug-resistant [XDR], pre-XDR-TB) from June 2018 to December 2022 in metropolitan Durban, South Africa. We collected named contacts and GPS coordinates of homes, clinics, and community locations visited regularly before diagnosis. Among participants genotypically clustered by whole genome sequencing (≤12 single-nucleotide polymorphisms), we quantified the proportion attributable to close versus casual contact. Close contact was defined as person-to-person links or overlapping hospitalizations. Casual contact links were based on geographic proximity of homes and community locations, or shared outpatient clinics. MEASUREMENTS AND MAIN RESULTS: We enrolled 305 of 383 (80%) persons diagnosed with second-line DR-TB. TB isolates were sequenced for 251 (83%) participants; 141 (56%) were genotypically linked, forming 25 clusters (range, 2-49 persons/cluster). Among clustered participants, 69 (49%) were epidemiologically linked by casual contact and 13 (9%) through close contact. Multivariable analysis identified living within 1 km (OR, 17.9), visiting proximate community locations (OR, 1.88), shared outpatient clinic (OR, 1.72), and person-to-person links (OR, 5.38) as significant risk factors associated with genotypic clustering. CONCLUSIONS: Casual contact in community locations accounted for half of transmission among genotypic clusters in a high-burden setting. Efforts to curb TB will require a greater emphasis on community-based measures to identify cases from casual contact or undetected intermediate cases, in addition to the current mainstay of contact tracing.