2025/01/01 by Prashant Sharma, Sakshi Dagariya, Gurvinder Singh +2 · 1 voice · 5 citations
Chemistry · Environmental Science · Materials Science · Medicine · #Biology #Chemistry #Composite material #Effects and risks of endocrine disrupting chemicals #Environmental chemistry #Environmental health #Fermentation #Food science #Human health #Lactobacillus #Lactobacillus rhamnosus #Materials science #Medicine #Microbiology #Microplastics #Microplastics and Plastic Pollution #Plastic pollution #Polyethylene #Polyethylene terephthalate #Toxicity #biodegradable polymer synthesis and properties
paper · pdf · doi:10.1039/d5na00613a
published in Nanoscale Advances 7(19), 6220-6238 (Royal Society of Chemistry)
openalex publication_date 2025/01/01 · openalex created_date 2025/08/04 · openalex updated_date 2026/07/27
viability, with pronounced effects after 16 days. Growth kinetics revealed impaired proliferation at higher concentrations, and confocal microscopy confirmed membrane damage. PBNPs also reduced antioxidant activity, antibacterial activity and increased biofilm formation, autoaggregation, and antibiotic sensitivity. Adhesion assays showed reduced bacterial attachment to colon epithelial cells, indicating disrupted colonization. Gene expression analysis reflected oxidative stress responses, while metabolomic profiling revealed alterations in energy, amino acid, and membrane lipid metabolism. In RBCs, PBNP exposure at higher concentrations induced morphological changes consistent with membrane destabilization, indicating potential hemolytic toxicity. In A549 cells, short-term exposure showed minimal effects, but prolonged exposure led to reduced viability, accompanied by DNA damage and increased expression of apoptotic, oxidative stress, and inflammatory markers. Metabolomic profiling revealed alterations in glucose metabolism, amino acid balance, and lipid-associated pathways. Ames testing showed no direct mutagenicity, but metabolic activation increased mutagenic potential, suggesting bioactivation-dependent genotoxicity. These findings demonstrate how real-world NPs can impair probiotic function, damage blood cells, and induce cellular toxicity, underscoring the need for deeper mechanistic understanding and appropriate regulatory strategies.