Ultra-mild bisulfite outperforms existing methods for 5-methylcytosine detection with low input DNA

2025/10/10 by Qing Dai, Tanner Baldwin, Ruitu Lyu +9 · 1 voice
Biochemistry, Genetics and Molecular Biology · #Epigenetics and DNA Methylation #Genomics and Phylogenetic Studies #RNA modifications and cancer

paper · pdf · doi:10.1101/2025.10.09.681456

openalex created_date 2025/10/10 · openalex publication_date 2025/10/10 · openalex updated_date 2026/07/14

Abstract

Abstract We present Ultra-Mild Bisulfite Sequencing (UMBS-seq), a method for 5-methylcytosine (5mC) detection that minimizes DNA degradation and background noise. UMBS-seq outperforms conventional bisulfite and enzymatic methyl-sequencing (EM-seq) methods in library yield, complexity, and conversion efficiency when applied to low-input DNA samples. In particular, its effectiveness with low-input cell-free DNA (cfDNA) and hybridization-based target capture highlight its potential for clinical applications, including 5mC biomarker detection and early disease diagnosis.

Discussions

• Ultra-mild bisulfite outperforms existing methods for 5-methylcytosine detection with low input DNA doi.org/10.1101/2025... [bsky, 0 points, 0 comments]

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