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Engineering prime editors in Salvia miltiorrhiza for precise genome modification

2025/08/26 by Qi Yao, Yi Ye, Muyao Yu +4 · 1 voice
Biochemistry, Genetics and Molecular Biology · #CRISPR and Genetic Engineering #Plant tissue culture and regeneration #Plant biochemistry and biosynthesis

paper · pdf · doi:10.1111/jipb.70006

openalex publication_date 2025/08/26 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

An optimized prime editing system in the model medicinal plant Danshen (Salvia miltiorrhiza) was developed, enabling base transversions, complex insertions and base substitutions. Precise editing of key genes generated germplasm with elevated contents of the bioactive abietane diterpene tanshinone, advancing synthetic biology applications in medicinal plants.

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