Engineering prime editors in Salvia miltiorrhiza for precise genome modification

2025/08/26 by Qi Yao, Yi Ye, Muyao Yu +4 · 1 voice · 3 citations
Biochemistry, Genetics and Molecular Biology · Mathematics · Medicine · #Biology #CRISPR and Genetic Engineering #Combinatorics #Computational biology #Gene #Genetics #Genome #Mathematics #Medicine #Plant biochemistry and biosynthesis #Plant tissue culture and regeneration #Prime (order theory) #Salvia miltiorrhiza

paper · pdf · doi:10.1111/jipb.70006

published in Journal of Integrative Plant Biology 68(4), 855-857 (Wiley)

openalex publication_date 2025/08/26 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

An optimized prime editing system in the model medicinal plant Danshen (Salvia miltiorrhiza) was developed, enabling base transversions, complex insertions and base substitutions. Precise editing of key genes generated germplasm with elevated contents of the bioactive abietane diterpene tanshinone, advancing synthetic biology applications in medicinal plants.

Citations

• Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

Discussions

• This #FreeAccess paper describes an optimized prime editing system in a model #medicinal #plant that allows precise editing of key #genes and generates germplasm with elevated amounts of tanshinone, a [bsky, 0 points, 0 comments]

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