Artificial Genetic Systems: Self‐Avoiding DNA in PCR and Multiplexed PCR

2010/06/28 by Shuichi Hoshika, Fei Chen, Nicole A. Leal +1 · 1 voice
Biochemistry, Genetics and Molecular Biology · #DNA and Nucleic Acid Chemistry #Advanced biosensing and bioanalysis techniques #Molecular Biology Techniques and Applications

paper · doi:10.1002/anie.201001977

openalex publication_date 2010/06/28 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/26

Abstract

Discerning in their choice of partner: The DNA of a self-avoiding molecular-recognition system (SAMRS) binds to natural DNA but not to members of the same SAMRS. Multiplexed PCR with an SAMRS based on 2-aminopurine (A*), 2-thiothymine (T*), 2′-hypoxanthine (G*), and N4-ethylcytosine (C*; see examples) showed the benefits of such systems for clinical analysis, which requires many DNA molecules to interact with target DNA but not with each other.

Citations

• Understanding Nucleic Acids Using Synthetic Chemistry

Discussions

• 3) Artificial Genetic Systems: Self-Avoiding DNA in PCR and Multiplexed PCR doi.org/10.1002/anie... 4) Artificially Expanded Genetic Information Systems for New Aptamer Technologies doi.org/10.3390/b [bsky, 0 points, 1 comments]