1990/12/01 by Praful Jani, Praful U. Jani, Gavin Halbert +4 · 5 citations
Biochemistry, Genetics and Molecular Biology · Medicine · #Carcinogens and Genotoxicity Assessment #Inhalation and Respiratory Drug Delivery
paper · doi:10.1111/j.2042-7158.1990.tb07033.x
crossref issued 1990/12/01 · crossref published 1990/12/01 · crossref published-online 1990/12/01 · crossref published-print 1990/12/01 · openalex publication_date 1990/12/01 · crossref created 2011/04/13 · crossref deposited 2024/10/31 · openalex created_date 2025/10/10 · crossref indexed 2026/08/01 · openalex updated_date 2026/08/01
Polystyrene microspheres in the size range 50 nm to 3 microns were fed by gavage to female Sprague Dawley rats daily for 10 days at a dose of 1.25 mg/kg-1. Previous histological evidence of the uptake of these particles and their absorption across the gastrointestinal tract and passage via the mesentery lymph supply and lymph nodes to the liver and spleen was confirmed by analysis of tissues for the presence of polystyrene by gel permeation chromatography. Measurement of radioactivity of tissues following administration of 100 nm and 1 micron I125-labelled polystyrene latex particles for 8 days was corroborative although less secure because of the potential lability of the labelled particles. The extent of absorption of 50 nm particles under the conditions of these experiments was 34% and of the 100 nm particles 26% (as measured by determination of polystyrene content), of which total, about 7% (50 nm) and 4% (100 nm), was in the liver, spleen, blood and bone marrow. Particles larger than 100 nm did not reach the bone marrow, and those larger than 300 nm were absent from blood. No particles were detected in heart or lung tissue.