2018/09/10 by Chandra Sekhar Yadavalli, Yadavalli Chandrasekhar, Garlapati Phani Kumar +6
Agricultural and Biological Sciences · Medicine · #GABA and Rice Research #Medicinal Plants and Neuroprotection #Phytochemicals and Medicinal Plants
paper · doi:10.1111/jphp.13007
openalex publication_date 2018/09/10 · crossref created 2018/09/10 · openalex created_date 2018/09/27 · crossref issued 2018/12/01 · crossref published 2018/12/01 · crossref published-online 2018/12/01 · crossref published-print 2018/12/01 · crossref deposited 2024/10/30 · openalex updated_date 2026/07/31 · crossref indexed 2026/08/02
Abstract Objectives This article investigates the anxiolytic activity of Terminalia chebula tannin-rich extract against picrotoxin (PTX; GABA antagonist)-induced anxiety in mice model. Methods Anxiolytic activity was studied by elevated plus maze (EPM), open field test (OFT), light/dark box test (LDT) and Vogel's conflict test (VCT). Electroencephalogram (EEG) was performed to know the changes in brain activity instigated by GABA antagonist. 5-hydroxytryptamine (5-HT), dopamine and norepinephrine levels in brain tissues were estimated by HPLC. The mRNA (CREB, BDNF, GABA, and 5-HT1A) and protein expression (CREB, p-CREB, BDNF, ERK ½, p-ERK ½, GABAARα1, 5-HT1A and GAPDH) levels in brain tissue were determined by RT-PCR and Western blot analysis, respectively. Key findings Terminalia chebula tannin-rich extract (TCHE) supplementation increased locomotion in mice towards open arm (EPM), time spent in illuminated area (LDT), rearing frequency (OFT) and number of shocks (VCT) compared to PTX (P < 0.05). Furthermore, TCHE down-regulated serum cortisol levels and showed increased levels of 5-HT, DA and NE. Gene expressions such as BDNF, CREB, GABAA and 5-HT1A were up-regulated by TCHE treatment compared to PTX. Conclusions Terminalia chebula tannin-rich extract showed significant anxiolytic activity against picrotoxin and could be used as natural therapy in neurodegenerative disorders.