2025/12/01 by Shihao Song, Jingyun Liu, Bing Wang +13 · 1 voice
Biochemistry, Genetics and Molecular Biology · Medicine · #Bacterial biofilms and quorum sensing #Cystic Fibrosis Research Advances #Infections and bacterial resistance
paper · pdf · doi:10.1002/mlf2.70044
openalex publication_date 2025/12/01 · openalex created_date 2025/12/26 · openalex updated_date 2026/08/01
Abstract Diffusible signal factor (DSF)‐family quorum sensing (QS) signals are widely utilized by many pathogenic bacteria to modulate various biological functions and virulence. Previous studies showed that cis ‐2‐decenoic acid ( cis ‐DA) is involved in the modulation of biofilm dispersion in Pseudomonas aeruginosa , but the regulatory mechanism is unclear. Here, we report that cis ‐DA regulates the physiology and virulence of P. aeruginosa through FadD1, a long‐chain fatty acid‐CoA ligase. cis ‐DA specifically binds to FadD1 and enhances the binding ability of FadD1 to the target gene promoter DNA regions. Further analysis showed that FadD1 is a global regulatory factor that controls the transcription of various target genes. Moreover, FadD1 showed catalytic activity on cis ‐2‐dodecenoic acid (BDSF) of Burkholderia cenocepacia and enhanced the competitiveness of P. aeruginosa . Together, our work presents a new DSF‐type QS signaling system in P. aeruginosa , which is highlighted by the signal receptor evolved from a canonical enzyme of fatty acid metabolism.