1989/01/01 by Purnima Singh, Shiv M. Singh, Masaharu Tsuji +4
Arts and Humanities · Biochemistry, Genetics and Molecular Biology · Environmental Science · Social Sciences · #Archaeological and Historical Studies #Classical Antiquity Studies #Classical Studies and Legal History #Polar Research and Ecology #Yeasts and Rust Fungi Studies
paper · doi:10.1016/j.cryobiol.2014.01.006
openalex publication_date 1989/01/01 · crossref created 2014/01/21 · crossref issued 2014/02/01 · crossref published 2014/02/01 · crossref published-print 2014/02/01 · crossref deposited 2019/08/06 · openalex created_date 2025/10/10 · openalex updated_date 2026/04/28 · crossref indexed 2026/07/31
A psychrophilic yeast species was isolated from glacier cryoconite holes of Svalbard. Nucleotide sequences of the strains were studied using D1/D2 domain, ITS region and partial sequences of mitochondrial cytochrome b gene. The strains belonged to a clade of psychrophilic yeasts, but showed marked differences from related species in the D1/D2 domain and biochemical characters. Effects of temperature, salt and media on growth of the cultures were also studied. Screening of the cultures for amylase, cellulase, protease, lipase, urease and catalase activities was carried out. The strains expressed high amylase and lipase activities. Freeze tolerance ability of the isolates indicated the formation of unique hexagonal ice crystal structures due to presence of 'antifreeze proteins' (AFPs). FAME analysis of cultures showed a unique trend of increase in unsaturated fatty acids with decrease in temperature. The major fatty acids recorded were oleic acid, linoleic acid, linolenic acid, palmitic acid, stearic acid, myristic acid and pentadecanoic acid. Based on sequence data and, physiological and morphological properties of the strains, we propose a novel species, Rhodotorula svalbardensis and designate strains MLB-I (CCP-II) and CRY-YB-1 (CBS 12863, JCM 19699, JCM 19700, MTCC 10952) as its type strains (Etymology: sval.bar.den'sis. N.L. fem. adj. svalbardensis pertaining to Svalbard).