1991/12/11 by Carl Michael Ruyter, Joachim H. H. Stöckigt, Joachim Stöckigt · 17 citations
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · Chemistry · #Alkaloid #Biochemistry #Cell culture #Chemistry #Cocoa and Sweet Potato Agronomy #Enzyme #Glucosyltransferase #Indole test #Plant tissue culture and regeneration #Plant-Microbe Interactions and Immunity #Stereochemistry #Substrate (aquarium) #Suspension culture #Transferase
paper · doi:10.1002/hlca.19910740811
published in Helvetica Chimica Acta 74(8), 1707-1712 (Wiley)
openalex publication_date 1991/12/11 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/21
Abstract The major alkaloid from Rauwolfia serpentina cell‐suspension cultures, the glucoalkaloid raucaffricine ( 2 ), was enzymatically formed from vomilenine ( 1 ) and UDPG in presence of microsomal‐bound enzyme. This glucosyltransferase exhibits a relatively high substrate specificity with strong preference for 1 and UDPG. The apparent K m values for 1 and UDPG were 40 μ M and 0.8 m M , respectively, for raucaffricine formation. Optimum transferase activity was observed at 50° and pH 6.3. The taxonomic distribution of this enzyme seems to be very limited because transferase can he exclusively detected in raucaffricine‐producing plant cells.