2022/03/22 by Carol Moreau, Frederick J. Warren, Tracey Rayner +4 · 1 voice
Agricultural and Biological Sciences · Nursing · #Food composition and properties #Genetic and Environmental Crop Studies #Legume Nitrogen Fixing Symbiosis
paper · pdf · doi:10.1016/j.carbpol.2022.119386
openalex publication_date 2022/03/22 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/03
A set of mutant pea lines carrying induced mutations within the major seed-expressed starch-branching enzyme gene has been characterised at the molecular, chemical and agronomic levels. Eight of the induced mutations, three of which predicted a premature stop codon, were compared with the naturally occurring starch-branching enzyme mutation within the same genetic background. Starch, amylose and sugar measurements, coupled with analysis by ultra-high performance liquid chromatography-size exclusion chromatography of starches, identified a range of phenotypes which were grouped according to the nature of the mutation. Homology modelling of proteins supported the differences in phenotypes observed. Differences in field performance were evident for selected mutants, particularly in seed yield and mean seed weight traits for early compared with late spring sowings. The data show the potential of an allelic series of mutants at this locus for nutritional studies. CHEMICAL COMPOUNDS: starch, amylose, amylopectin, raffinose, stachyose, verbascose.