2004/01/01 by Ildenize Barbosa da Silva Cunha, Kelly Salomão, M. T. Shimizu +9
Agricultural and Biological Sciences · Immunology and Microbiology · #Bee Products Chemical Analysis #Insect and Pesticide Research #Toxin Mechanisms and Immunotoxins
paper · pdf · doi:10.1248/cpb.52.602
crossref issued 2004/01/01 · crossref published 2004/01/01 · crossref published-print 2004/01/01 · openalex publication_date 2004/01/01 · crossref created 2004/05/06 · crossref deposited 2021/03/18 · openalex created_date 2025/10/10 · crossref indexed 2026/07/30 · openalex updated_date 2026/08/01
Extracts from different samples of Brazilian propolis were obtained by Soxhlet extraction or maceration at room temperature using ethanol, water, and accombination of both solvents. Analysis of their composition using HPLC revealed that no major differences were seen when a propolis sample was subject to different extraction methods. The activity of the 15 extracts was assayed against bloodstream trypomastigotes of Trypanosoma cruzi, the etiologic agent of Chagas' disease. Multivariate analysis was applied to evaluate the efficiency of the different extracts and the trypanocidal activity. The extracts could be divided into two groups. In the first, in which, extracts were obtained by reflux in Soxhlet using 100% ethanol, there was a lower content of bioactive compounds and consequently lower trypanocidal activity. Extract 136-Et100 stands out in this group, since it had the highest levels of bioactive compounds together with highest activity against the parasite when compared with all other extracts. The second group comprises extracts with intermediate levels of bioactive compounds and higher activity against T. cruzi.