2022/02/18 by Mohammed Al‐Rasheed, M. Al-Rasheed, Christopher Ball +6
Agricultural and Biological Sciences · Immunology and Microbiology · Medicine · #Animal Virus Infections Studies #Microbial infections and disease research #Viral gastroenteritis research and epidemiology
paper · doi:10.1080/00071668.2022.2035675
openalex publication_date 2022/02/18 · crossref created 2022/02/18 · crossref issued 2022/06/30 · crossref published 2022/06/30 · crossref published-online 2022/06/30 · crossref published-print 2022/07/04 · crossref deposited 2023/01/27 · openalex created_date 2025/10/10 · crossref indexed 2026/07/28 · openalex updated_date 2026/07/29
1. The role of the Harderian gland (HG), choanal cleft (CC) and turbinate in terms of IBV M41 viral load compared to the trachea, and immune (innate, cellular and mucosal) responses were studied in 21-day-old commercial broiler chickens.2. After virulent IBV M41 challenge, the antigen concentration detected either by quantitative RT-PCR or immunohistochemistry peaked at 2–3 days post challenge (dpc) in all tissues. Significant increases of lachrymal IBV-specific IgA and IgY levels were found at 4–5 dpc.3. Gene transcription showed a significant up-regulation of TLR3, MDA5, IL-6, IFN-α and IFN-β, where patterns and magnitude fold-change of mRNA transcription were dependent on the gene and tissue type.4. The results demonstrated active IBV M41 replication in the HG, CC and turbinate, comparable to levels of replication found in the trachea. Data on immune-related genes in head-associated tissues provide further understanding on the immunobiology of IBV and offer opportunities to identify their use as quantitative biomarkers in pathogenicity and vaccination-challenge studies.