2010/08/01 by M.K. Turkyilmaz, Mehmet Kenan Türkyılmaz, L. Karagenc +3 · 2 citations
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Animal Behavior and Reproduction #Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities #Genetic and phenotypic traits in livestock
paper · doi:10.1080/00071668.2010.502521
crossref issued 2010/08/01 · crossref published 2010/08/01 · crossref published-print 2010/08/01 · openalex publication_date 2010/08/01 · crossref created 2010/10/05 · crossref deposited 2019/06/05 · openalex created_date 2025/10/10 · crossref indexed 2026/07/30 · openalex updated_date 2026/07/31
1. The aim of the present study was to determine the sex of newly-hatched chicks of Denizli chicken, a local Turkish breed, by polymerase chain reaction (PCR) using DNA extracted from the chorioallantoic membrane (CAM). 2. Fertilised eggs were incubated individually and a total of 20 CAM samples were collected following the hatching process. DNA was isolated from the CAM samples and PCR was performed using W-repeat (W) and 18 S ribosomal gene (R) primers. 3. Screening of the PCR products by agarose gel electrophoresis revealed that males have a single band (256 bp) and females have an extra second band (415 bp) as expected. 4. The present study describes a reliable, rapid, and simple multiplex PCR protocol that can be put into use to sex local breeds of chicken in which phenotypic sexing is impossible, using DNA isolated from the CAM that is discarded and remains attached to the egg shell following the hatching process.