2001/03/01 by Michael Clinton, M. Clinton, L. Haines +3 · 2 citations
Biochemistry, Genetics and Molecular Biology · #Animal Genetics and Reproduction #Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities #Genetic and phenotypic traits in livestock
paper · doi:10.1080/713655025
crossref issued 2001/03/01 · crossref published 2001/03/01 · crossref published-print 2001/03/01 · openalex publication_date 2001/03/01 · crossref created 2007/06/25 · crossref deposited 2019/04/29 · openalex created_date 2025/10/10 · crossref indexed 2026/07/30 · openalex updated_date 2026/07/31
1. Analysis of gene expression in the developing chick gonads requires the collection of male and female tissues from embryos between 3.5 d and 8.5 d of development. However, male and female chick embryos are indistinguishable by morphological examination before d 7.5 of development. 2. Sex identification of earlier embryos is only possible by molecular methods, which at present are laborious and time consuming. 3. We have devised a PCR-based sexing protocol which combines both sex specific and control reactions in a single tube assay. The assay is rapid and effective over a wide range of DNA concentrations and is tolerant of poor quality DNA. 4. Procedures are described for identifying the sex of individual embryos using either tissue samples or a small number of cells recovered from amniotic fluid.