2017/06/22 by Beibei Song, Huijun He, Lufeng Chen +4
Chemistry · Environmental Science · #Analytical chemistry methods development #Environmental Toxicology and Ecotoxicology #Mercury impact and mitigation studies
paper · doi:10.1080/00032719.2016.1269119
crossref issued 2017/06/22 · crossref published 2017/06/22 · crossref published-online 2017/06/22 · openalex publication_date 2017/06/22 · crossref created 2017/06/22 · crossref published-print 2017/09/02 · crossref deposited 2017/09/11 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/22 · crossref indexed 2026/07/29
Species-specific stable isotope dilution in combination with gold trap- or gas chromatography (GC)-inductively coupled plasma mass spectrometry (ICP-MS) is reported for the determination of inorganic mercury and methylmercury in diatoms (Chaetoceros curvisetus). The optimum conditions for the separation parameters were established. The isotope dilution analysis was performed using 199Hg-enriched Hg2+ and laboratory-synthesized 201Hg-enriched methylmercury. The absolute detection limits obtained with isotope dilution-ICP-MS were 9 pg for total mercury and 0.6 pg for methylmercury. The relative error of 7 Hg isotopic abundances based on the peak area measurements was better than 2.0% for 20 pg of methylmercury (as Hg) and 250 pg of inorganic mercury. The accuracy of the method was validated with a biological certified reference material. The developed method was then applied to investigate the uptake of inorganic mercury and methylmercury by C. curvisetus. Continuous uptake of inorganic mercury and methylmercury was observed during 5 days of incubation.