An enzymatic microfluidic sensor for β-lactam antibiotics based on cross-linked ancestral β-lactamase crystals

2026/01/01 by Isaac Rodríguez-Ruiz, Pablo M Garrido, Valeria A. Risso +4 · 1 voice
Biochemistry, Genetics and Molecular Biology · Engineering · #Antibiotic Resistance in Bacteria #Bacterial Identification and Susceptibility Testing #Biosensors and Analytical Detection

paper · pdf · doi:10.1039/d5na00906e

openalex publication_date 2026/01/01 · openalex created_date 2026/01/23 · openalex updated_date 2026/07/22

Abstract

, its capability to degrade a variety of lactam antibiotics, including man-made third-generation antibiotics, broadens its potential application. Full catalytic activity of the ancestral enzyme was retained after immobilization, and inhibition by antibiotics such as ampicillin and sulbactam was detected at concentrations as low as parts per billion. These results support the use of ancient enzymes as stable and responsive biorecognition elements in cost-effective, high-throughput analytical systems targeting environmental pollutants and pharmaceutical compounds.

Discussions

• Check out this article in Nanoscale Advances from José A. Gavira et al. 👇 ppb-level detection of β-lactam antibiotics in a microfluidic chip loaded with ancestral β-lactamase CLECs Read the full art [bsky, 0 points, 0 comments]

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