Terahertz dielectric assay of solution phase protein binding

2007/06/11 by Jing–Yin Chen, J. R. Knab, Shuji Ye +2 · 1 voice · 1 citation
Chemistry · Engineering · Medicine · #Monoclonal and Polyclonal Antibodies Research #Terahertz technology and applications #thermodynamics and calorimetric analyses

paper · doi:10.1063/1.2748852

openalex publication_date 2007/06/11 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/27

Abstract

The authors demonstrate a method for rapid determination of protein-ligand binding on solution phase samples using terahertz dielectric spectroscopy. Measurements were performed using terahertz time domain spectroscopy on aqueous solutions below the liquid-solid transition for water. Small ligand binding sensitivity was demonstrated using triacetylglucosamine and hen egg white lysozyme with a decrease in dielectric response with binding. The magnitude of the change increases with frequency.

Cited by

• Quasi-Optical Sub-THz Circular Dichroism Spectroscopy of Solvated Myoglobin

Discussions

• 2/3 Chen et al. (2007) measured differential terahertz transmission through biotin-avidin complexes in aqueous solution at GHz frequencies. https://doi.org/10.1063/1.2748852 [bsky, 0 points, 1 comments]

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