Spatial Imaging and Quantification of Hydrogen Peroxide in <em>Arabidopsis</em> Roots: From Sample Preparation to Image Analysis

2026/01/01 by Mario Fenech, V Amorim-Silva · 1 voice
Agricultural and Biological Sciences · #Plant responses to water stress #Plant Stress Responses and Tolerance #Plant nutrient uptake and metabolism

paper · doi:10.21769/bioprotoc.5659

openalex publication_date 2026/01/01 · openalex created_date 2026/03/24 · openalex updated_date 2026/07/02

Abstract

measurement in DAB-stained roots using the open-source software FIJI/ImageJ. • Provides detailed, step-by-step video tutorials for image analysis in FIJI/ImageJ. • Includes a Fiji/ImageJ script (Macro 1) for automating the application of fixed-intensity scaling using Spectrum LUT.

Citations

• The fluorescent protein sensor ro<scp>GFP</scp>2‐Orp1 monitors <i>in vivo</i> H₂O₂ and thiol redox integration and elucidates intracellular H₂O₂ dynamics during elicitor‐induced oxidative burst in Arabidopsis
• Estimation of hydrogen peroxide in plant extracts using titanium(IV)
• Multiple Comparisons Using Rank Sums
• Use of Ranks in One-Criterion Variance Analysis
• On a Test of Whether one of Two Random Variables is Stochastically Larger than the Other

Discussions

• Spatial Imaging and Quantification of Hydrogen Peroxide in Arabidopsis Roots: From Sample Preparation to Image Analysis Bio Protocol 👉 doi.org/10.21769/Bio... [bsky, 0 points, 1 comments]

Related

• Methodology to enable high-throughput imaging of Arabidopsis seedlings on cover glass-bottom multiwell plates
• MultipleXLab: A high-throughput portable live-imaging root phenotyping platform using deep learning and computer vision
• Application of ultra-weak photon emission imaging in plant stress assessment
• Distribution of superoxide and hydrogen peroxide in <i>Arabidopsis</i> root and their influence on root development: possible interaction with peroxidases
• Plant Doctor: A hybrid machine learning and image segmentation software to quantify plant damage in video footage