Engineered pegRNAs improve prime editing efficiency

2021/10/04 by James W. Nelson, Peyton B. Randolph, Simon P. Shen +9 · 1 voice · 13 citations
Biochemistry, Genetics and Molecular Biology · #Advanced biosensing and bioanalysis techniques #CRISPR and Genetic Engineering #RNA and protein synthesis mechanisms

paper · doi:10.1038/s41587-021-01039-7

crossref issued 2021/10/04 · crossref published 2021/10/04 · crossref published-online 2021/10/04 · openalex publication_date 2021/10/04 · crossref created 2021/10/04 · crossref published-print 2022/03/01 · crossref deposited 2023/02/07 · openalex created_date 2025/10/10 · crossref indexed 2026/07/30 · openalex updated_date 2026/07/31

Citations

• Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

Cited by

• Therapeutic in vivo delivery of gene editing agents
• Dual function of ERH in primary miRNA biogenesis
• Prime assembly with linear DNA donors enables large genomic insertions
• Development of programmable RNA imaging with RNA-guided GFP via click chemistry
• RNA-LNP–mediated in vivo prime editing corrects disease phenotypes in a mouse model of citrullinemia type I
• Prime editing of a pathogenic Scn1a allele ameliorates seizure phenotypes in a GEFS + mouse model
• In Vivo Prime Editing by Lipid Nanoparticle Co-Delivery of Chemically Modified pegRNA and Prime Editor mRNA
• Treating genetic blood disorders in the era of CRISPR-mediated genome editing
• From Gene Knockouts to Genome Remodeling: Large DNA Fragment Deletion Technologies in Plants
• Functional phenotyping of genomic variants using joint multiomic single-cell DNA–RNA sequencing
• De-extinction technology and its application to conservation
• Prime editing: advances and therapeutic applications
• Design of CoQ10 crops based on evolutionary history

Discussions

• doi.org/10.1038/s415... [bsky, 1 points, 1 comments]