2024/01/01 by Amanda D. Roe, Ashlyn A. Wardlaw, Skye Butterson +1 · 1 voice · 1 citation
Environmental Science · Medicine · #Adipose Tissue and Metabolism #Physiological and biochemical adaptations #Thermal Regulation in Medicine
paper · doi:10.1016/j.cris.2024.100073
openalex publication_date 2024/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/22
Diapause is a form of internally-controlled dormancy that allows insects to avoid stressful conditions and periods of low food availability. Eastern spruce budworm (Choristoneura fumiferana Clemens), like many cold-adapted insects, enter diapause well in advance of winter conditions, thus exposing them to elevated temperatures during fall that can deplete energy stores and impact post-diapause survival. We explored the impact of fall conditions on C. fumiferana by manipulating the length of the fall period and exposure temperatures during the diapause initiation phase of second instar larvae in a factorial design. We exposed second instar larvae to four fall temperatures (10, 15, 20, and 25°C) and five exposure times (1, 2, 4, 6, and 10 weeks) prior to standardized diapause conditions. We measured metabolites (glycogen, glycerol, and protein) prior to and during diapause for a subset of individuals. We also measured post-diapause survival by quantifying emergence following diapause conditions for a subset of individuals. We found that long, warm fall conditions depleted glycogen content and lowered post-diapause survival. We also found that short, cool conditions impacted post-diapause survival, although glycogen content remained high. Our results show that fall conditions have substantial fitness consequences to overwintering insects. Optimal fall conditions struck a balance between exposure time and temperature. Our findings point to a potentially adaptive reason for early diapause onset: that an undescribed, but temperature-sensitive process is occurring in C. fumiferana larvae during the diapause initiation period that is essential for overwintering survival and post-diapause emergence.