The consequence of mismatched buffers in purity checks when spectral cell sorting

2024/11/30 by Rita A. S. Dapaah, Laura Ferrer‐Font, Xiaoshan Shi +7 · 1 voice
Biochemistry, Genetics and Molecular Biology · Engineering · #Cell Image Analysis Techniques #Microfluidic and Bio-sensing Technologies #Single-cell and spatial transcriptomics

paper · pdf · doi:10.1002/cyto.a.24911

openalex publication_date 2024/11/30 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/04

Abstract

Although spectral flow cytometry has become a ubiquitous tool for cell analysis, the use of spectral cytometry on cell sorters requires additional considerations arising from the unique requirements of sorting workflows. Here, we show that care should be taken when ascertaining the purity of a sort on a spectral cell sorter, as the mismatch of buffers used for initial sample suspension and the buffers used for sort collection can affect the unmixing of the data, potentially giving rise to erroneous purity check results.

Discussions

• How important are buffers in spectral sorting? Check out this great paper that was recently published in #CytometryPartA by Rita Dapaah doi.org/10.1002/cyto... [bsky, 1 points, 0 comments]

Related

• Uncertainty quantification in flow cytometry using a cell sorter
• AutoSpectral improves spectral flow cytometry accuracy through optimised spectral unmixing and autofluorescence-matching at the cellular level
• An evaluation of sorter induced cell stress (SICS) on peripheral blood mononuclear cells (PBMCs) after different sort conditions - Are your sorted cells getting SICS?
• CytoBatchFlagR : A Comprehensive Framework to Objectively Assess High‐Parameter Cytometry Data for Batch Effects
• Supervised Contamination Detection, with Flow Cytometry Application