2024/09/24 by Daekee Kwon, Bo Kyung Moon, Mijung Han +3 · 1 voice
Engineering · Immunology and Microbiology · Medicine · #CAR-T cell therapy research #Immune Cell Function and Interaction #Nanowire Synthesis and Applications
paper · doi:10.1016/j.omton.2024.200885
openalex publication_date 2024/09/24 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
Chimeric antigen receptor (CAR)-T cell treatment is an innovative drug with excellent therapeutic effects against B cell blood cancer. However, multiple side effects and ultra-high treatment costs must be overcome. Off-the-shelf CAR natural killer (NK) cells can be a good alternative to patient-specific CAR-T cells. The purpose of this study was to combine cellular reprogramming, gene editing, and differentiation technologies to produce full-off-the-shelf NK cells and to verify their efficacy and safety. Genetically stable universal and potent CAR (upCAR)-induced pluripotent stem cells (iPSCs) showed biallelic insertions and deletions in the coding sequence and no off-target effects. upCAR-NK cells showed a very high differentiation yield and in vitro proliferation, and freezing/thawing was possible. In addition, upCAR-NK cells secrete interferon-γ when they meet cancer cells, showing cytotoxic effects in vitro and in vivo . upCAR-NK cells show no obvious toxicity in vivo . In conclusion, this study developed genetically stable upCAR-iPSCs and upCAR-NK cell platform technologies that are less likely to have side effects and can be more economically developed for B cell blood cancer than CAR-T cells. In the future, this technology could be useful in developing a full-off-the-shelf CAR-NK cells anti-cancer immune cell therapy with low side effects, high efficacy, and a low price. Kang and colleagues developed genetically stable upCAR-iPSCs and upCAR-NK cells platform technologies by combining cellular reprogramming, gene editing, and differentiation technologies. This platform could be useful in developing a full-off-the-shelf anti-cancer immune cell therapy with low side effects, high efficacy, and a low price.