2025/03/30 by Sara Campos, Uxía Rodriguez, Jesús S. Troncoso +1 · 1 voice · 1 citation
Earth and Planetary Sciences · Biochemistry, Genetics and Molecular Biology · Agricultural and Biological Sciences · #Marine and coastal plant biology #Reproductive biology and impacts on aquatic species #Aquaculture Nutrition and Growth
paper · doi:10.1016/j.cryobiol.2025.105235
openalex publication_date 2025/03/30 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/22
This study investigates the challenges and potential solutions for cryopreserving sea urchin ( Paracentrotus lividus ) eggs. It focuses on understanding the thresholds and osmoregulatory mechanisms, cryoprotectant toxicity, and cold tolerance in these eggs, which are especially difficult to preserve due to high sensitivity to cryoprotectants and low-temperature injury. Key findings suggest that stepwise addition of cryoprotectants, particularly dimethyl sulfoxide (Me 2 SO) combined with other agents like DMF or methanol, reduces toxicity and enhances larval survival. The study also highlights that short-term exposure to low temperatures (4 °C) minimally impacts egg viability, offering insights for optimizing cooling protocols. By examining osmolarity, salinity, and cryoprotectant interactions, the research proposes vitrification as a promising cryopreservation technique. These findings will contribute to refining current embryo cryopreservation protocols and provides information for the future vitrification of sea urchin eggs, with implications for marine conservation and aquaculture.