2025/01/01 by Jocky C. K. Kung, Alan Kádek, Knut Kölbel +28 · 1 voice
Chemistry · Physics and Astronomy · #Analytical Chemistry and Chromatography #Mass Spectrometry Techniques and Applications #Nuclear Physics and Applications
paper · pdf · doi:10.1039/d5cp00604j
openalex publication_date 2025/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/30
nano-electrospray ionization and subsequently probed by extreme ultraviolet (FLASH2) or soft X-ray (PETRA III) radiation, in either their folded state or following collision-induced activation in the gas phase. Depending on the size of the biomolecule and the activation method, protein fragmentation, dissociation, or enhanced ionization were observed. Additionally, an extension of the setup by ion mobility is described, which can serve as a powerful tool for structural separation of biomolecules prior to X-ray probing. The first experimental results are discussed in the broader context of current and upcoming X-ray sources, highlighting their potential for advancing structural biology in the future.