2025/08/23 by Ronald Van Eyken, Sam Jordens, Dries Oome +2 · 1 voice
Biochemistry, Genetics and Molecular Biology · Materials Science · #Enzyme Structure and Function #Microbial Metabolic Engineering and Bioproduction #Protein Structure and Dynamics
paper · pdf · doi:10.1101/2025.08.23.671898
openalex publication_date 2025/08/23 · openalex created_date 2025/08/24 · openalex updated_date 2026/07/14
ABSTRACT Uncurbed exponential proliferation might not always be required for genetically modified microorganisms, and might even cause unpredictable liabilities in their behavior and impact. We therefore constructed a bacterial chassis that adheres to linear proliferation for a finite number of generations, based on the asymmetric segregation of a gradually declining intracellular protein aggregate that is engineered to produce a conditionally essential metabolite such as cAMP.