1929/01/01 by Marta A. Garbacz, Scott A. Lujan, Adam Burkholder +6 · 1 voice · 1 citation
Arts and Humanities · Biochemistry, Genetics and Molecular Biology · #Archaeological and Geological Studies #Archaeological and Historical Studies #DNA Repair Mechanisms #Fungal and yeast genetics research #Genomics and Chromatin Dynamics #Medieval Architecture and Archaeology
paper · pdf · doi:10.1038/s41467-018-03270-4
openalex publication_date 1929/01/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/06/24
To investigate nuclear DNA replication enzymology in vivo, we have studied Saccharomyces cerevisiae strains containing a pol2-16 mutation that inactivates the catalytic activities of DNA polymerase ε (Pol ε). Although pol2-16 mutants survive, they present very tiny spore colonies, increased doubling time, larger than normal cells, aberrant nuclei, and rapid acquisition of suppressor mutations. These phenotypes reveal a severe growth defect that is distinct from that of strains that lack only Pol ε proofreading (pol2-4), consistent with the idea that Pol ε is the major leading-strand polymerase used for unstressed DNA replication. Ribonucleotides are incorporated into the pol2-16 genome in patterns consistent with leading-strand replication by Pol δ when Pol ε is absent. More importantly, ribonucleotide distributions at replication origins suggest that in strains encoding all three replicases, Pol δ contributes to initiation of leading-strand replication. We describe two possible models.