2025/10/01 by Sonia Albillos‐Arenal, Javier Alonso‐del‐Real, Ana Cristina Adam +2 · 1 voice
Biochemistry, Genetics and Molecular Biology · Engineering · Agricultural and Biological Sciences · #Fungal and yeast genetics research #Biofuel production and bioconversion #Fermentation and Sensory Analysis
paper · pdf · doi:10.1111/1751-7915.70244
openalex publication_date 2025/10/01 · openalex created_date 2025/10/12 · openalex updated_date 2026/07/29
Ethanol stress poses a considerable challenge for Saccharomyces cerevisiae during fermentation. Strains carrying an extra copy of chromosome III exhibit enhanced ethanol tolerance. Here, we investigated the underlying mechanisms of this tolerance, focusing on gene dosage effects and differential gene expression under ethanol stress. We compared the gene expression profiles of a strain with three copies of chromosome III and its derivative with two copies, exposed to 6% and 10% ethanol. Our analysis identified TUP1, located on chromosome III, as a key regulator of the ethanol stress response. Deleting one copy of TUP1 in the tolerant strain diminished its ethanol tolerance, suggesting that chromosome III aneuploidy in ethanol-tolerant strains enhances adaptive responses by increasing TUP1 copy number. Our findings offer insights into the genetic basis of ethanol tolerance, with potential applications for optimising industrial fermentation processes and understanding the role of aneuploidy in the domestication of industrial yeasts.