2026/02/19 by Chi‐Hun Park, Young-Hee Jeoung, JiTao Wang +1 · 1 voice
Medicine · #Reproductive Biology and Fertility #Growth Hormone and Insulin-like Growth Factors #Ovarian function and disorders
paper · pdf · doi:10.3389/fcell.2026.1783082
openalex publication_date 2026/02/19 · openalex created_date 2026/02/19 · openalex updated_date 2026/07/23
Insulin-like growth factor 1 (IGF1) signaling is a conserved regulator of embryonic growth and survival. However, the specific role of IGF1 signaling mediated by its cognate receptor IGF1R during mammalian preimplantation development remains unclear and unexplored. In this study, we employed both genetic ablation using cytidine deaminase base editors and pharmacological inhibition to assess the role of IGF1R in porcine early embryonic development. Embryos lacking IGF1R advanced through early cleavage divisions and progressed to blastocyst formation; however, they displayed delayed blastocyst development and significantly increased apoptosis. Lineage segregation was largely unperturbed. Exogenous IGF-1 supplementation did not ameliorate developmental impairments in IGF1R -knockout embryos and instead exacerbated apoptotic responses when receptor signaling was compromised. Collectively, these results establish that IGF1R signaling is dispensable for cell fate specification but is crucial for regulating blastocyst growth dynamics and embryonic viability.