2025/01/01 by Mohsen A. Khormi, Mostafa Y. Abdel‐Glil, Hanan M. Alharbi +6 · 1 voice · 1 citation
Agricultural and Biological Sciences · Chemistry · #Moringa oleifera research and applications #Heavy Metals in Plants #Bee Products Chemical Analysis
paper · doi:10.1515/gps-2025-0087
openalex publication_date 2025/01/01 · openalex created_date 2025/10/15 · openalex updated_date 2026/07/16
Abstract The current study successfully biosynthesized bimetallic silver–selenium nanoparticles (Ag–Se NPs) using an extract from Salvia hispanica seeds. Our research revealed that Salvia hispanica seed extract is a substantial source of various phytochemicals. Ag–Se NPs were characterized by UV, XRD, FTIR, HR-TEM, SEM-EDX analyses, and mapping studies. Moreover, Ag–Se NPs showed antimicrobial activity against E. coli, K. pneumoniae, P. aeruginosa, S. aureus , and B. subtilis . In addition, Ag–Se NPs demonstrated antibiofilm effectiveness against two biofilm-forming bacteria, K. pneumoniae and S. aureus. Ag–Se NPs exhibited significant antioxidant activity in the DPPH and ABTS experiments, surpassing ascorbic acid (IC 50 = 354 and 241 µg·mL −1 ). In contrast, the reported low IC 50 values for the tested Ag–Se NPs against prostate (PC3), and ovarian (SK-OV3) cancerous cell lines were 52.5 and 62.94 μg·mL −1 , respectively, indicating their significant efficacy against these cancerous cell lines, and the IC 50 for the Vero cells was 187.8 µg·mL −1 . Anti-diabetic effects were demonstrated by the inhibition of α-amylase (91.1%) and α-glucosidase (85.6%) at 1 mg·mL −1 . Ultimately, Ag–Se NP dosage at the MIC values exhibited reduced expression of the virulence genes mag A and tox A in K. pneumoniae and P. aeruginosa by 29.4% and 24.5%, respectively.