2025/09/25 by William Scott, Richard C. Knighton, Mohan K. Balasubramanian +1 · 1 voice
Biochemistry, Genetics and Molecular Biology · Chemistry · #Advanced Polymer Synthesis and Characterization #Chemical Synthesis and Analysis #Photopolymerization techniques and applications
paper · doi:10.26434/chemrxiv-2025-h6sfb
openalex publication_date 2025/09/25 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/14
Click reactions between strained alkyne and azide groups allow bioconjugation of fluorescent dyes onto proteins bearing unnatural amino acids. Strained alkyne dyes for protein imaging are available but often expensive. Here we report the simple conjugation of the strained alkynes bicyclo[6.1.0]nonyne (BCN) and dibenzocyclooctyne (DIBO) with rhodamine B and fluorescein fluorescent groups, with the goal of establishing an easy, accessible and rapid method for making a range of strained alkyne dyes. One known and three novel dyes were generated and validated through click reactions with azide-bearing proteins. Overall, this conjugation approach is an efficient, low cost and rapid way of producing clickable protein dyes that are otherwise costly