Optimization of AsCas12a for combinatorial genetic screens in human cells

2020/07/13 by Peter C. DeWeirdt, Kendall R Sanson, Kendall R. Sanson +15 · 1 voice · 5 citations
Biochemistry, Genetics and Molecular Biology · #Advanced biosensing and bioanalysis techniques #CRISPR and Genetic Engineering #RNA and protein synthesis mechanisms

paper · doi:10.1038/s41587-020-0600-6

crossref issued 2020/07/13 · crossref published 2020/07/13 · crossref published-online 2020/07/13 · openalex publication_date 2020/07/13 · crossref created 2020/07/13 · crossref published-print 2021/01/01 · crossref deposited 2023/05/20 · openalex created_date 2025/10/10 · crossref indexed 2026/07/23 · openalex updated_date 2026/07/28

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Discussions

• This looks really cool! Would you consider including variable direct repeats, this should minimize homology between the interspersed repeats? >>> doi.org/10.1038/s415... [bsky, 1 points, 0 comments]