2025/11/11 by Byungchan Kim, Alexander C. Howard, Tom Y. Cheng +11 · 1 voice
Medicine · Engineering · #Optical Imaging and Spectroscopy Techniques #Optical Coherence Tomography Applications #Photoacoustic and Ultrasonic Imaging
paper · pdf · doi:10.1038/s42003-025-08915-x
openalex publication_date 2025/11/11 · openalex created_date 2025/11/11 · openalex updated_date 2026/07/23
Recently, speckle contrast optical spectroscopy (SCOS) enabled non-invasive, high signal-to-noise-ratio (SNR) human cerebral blood flow (CBF) measurements, relevant for both neuroscience and clinical monitoring of diseases with CBF dysregulation. Single-channel SCOS measurements limit the information obtained to only one location on the head. In this work, we develop a multi-channel SCOS system to map spatial heterogeneity in CBF changes during human brain activation. Using a galvanometer, we temporally multiplex a free-space laser to 7 source fibers positioned at different locations on the head. Diffuse light collected from the tissue is captured by fiber bundles projecting to 17 complementary metal-oxide semiconductor (CMOS) cameras, resulting in 50 source-detector channels measuring optical density (OD) and relative CBF changes covering an area of 7.6 cm by 6.6 cm on the head. We validate the spatial specificity and stability of the system using a liquid flow phantom. We then measure brain activity during a word-color Stroop task in 15 subjects and obtain brain activation maps. The average signal changes in the channel showing the largest activation is 1.7× 10-2 in ΔOD and 6.6% in CBF. We present a high-density, multi-channel speckle contrast optical spectroscopy system for mapping cerebral blood flow in humans, enabling noninvasive brain function measurements